阻塞性睡眠呼吸暂停(OSA)是一种慢性疾病,主要表现为睡眠时空气流动减少或完全停止。它发生在支撑喉咙软组织的肌肉在睡眠时放松,导致上呼吸道狭窄或闭合。睡眠呼吸暂停是一种严重的疾病,心血管并发症的风险增加,生活质量受损。持续气道正压通气(Continuous positive airway pressure, CPAP)是治疗中重度阻塞性睡眠呼吸暂停(OSA)最有效的方法,对治疗轻度睡眠呼吸暂停也有效。然而,CPAP治疗与一些鼻腔副作用的发展有关,对使用者来说不方便,导致依从率低。CPAP治疗对上呼吸系统的影响及副作用的发病机制尚不完全了解,也没有充分的研究。为了更好地了解CPAP治疗对上呼吸道系统的影响,我们开发了一个体外3d打印微流控平台。然后将鼻上皮细胞系RPMI 2650暴露在特定的条件下模拟体内环境。为了创造这些条件,利用微流控装置暴露在气液界面生长和分化的鼻上皮细胞。气流类似于CPAP,压力范围在0-20厘米H20之间。 Cells exposed to pressure showed decreased barrier integrity, change in cellular shape, and increased cell death (lactate dehydrogenase release into media) compared to unstressed cells. Stressed cells also showed increased secretions of inflammatory markers IL-6 and IL-8 and had increased production of ATP. Our results suggest that stress induced by airflow leads to structural, metabolic, and inflammatory changes in the nasal epithelium, which may be responsible for developing nasal side-effects following CPAP treatment.

参考文献

PubMed