硫化物硫化物(HGS)经常包含在阿育吠陀,藏族和中药中,以协助推定的治疗效果,但民族科医生仍然难以捉摸。The present study examined the protective effects of α-HgS-containing Hua-Feng-Dan and β-HgS-containing 70 Wei-Zhen-Zhu-Wan (70W, Rannasangpei) against Parkinson’s disease mice induced by lipopolysaccharide (LPS) plus 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).
对成年雄性C57BL / 6小鼠的单一注射LPS(5mg / kg IP),150天后,给出了低剂量的MPTP(15mg / kg,IP,4天)产生“two-hit” Parkinson’s disease model. Together with MPTP treatment, mice were fed with clinically-relevant doses of Hua-Feng-Dan (0.6 g/kg) and 70W (0.2 g/kg) for 35 days. Rotarod test was performed to examine muscle coordination capability. At the end of the experiment, brain was transcardially perfused with paraformaldehyde, the substantia nigra was sectioned for microglia (Iba1 staining) and dopaminergic neuron (THir staining) determination. Colon bacterial DNA was extracted and subjected to qPCR analysis with 16S rRNA probes.
由LPS产生的低级,慢性神经炎性炎症加重MPTP神经毒性,如电机活性降低所证明,增强的小胶质细胞活化和多巴胺能神经元的丧失。华丰丹和70W增加了旋流活动,并改善了大脑中的病理病变。在肠道微生物体中,LPS Plus MPTP增加了Verrucomicrobiaceae,Methanobacteriaceae,Pronicromonosporaceae和Hua-Feng-Dan和70W衰减了蛋白酶菌。
含α-HGS的华风DAN和含有70W的β-HGS在临床剂量下保护免受慢性LPS加上MPTP诱导的脑部和肠道的毒性,表明含Hgs的传统药物可以将肠道微生物征靶向其治疗效果。

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